Plasmid

Part:BBa_K4354011

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-29)


plasmid pUC19

Plasmid pUC19 is a common vector from E.coli. According to literature and some experiment, we validate that it can replicate itself in Bacillus subtilis. As a medium-high copy vector, it is used to express EL222, a photo-sensitive protein, in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 423
    Illegal PstI site found at 435
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal PstI site found at 435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 417
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 423
    Illegal PstI site found at 435
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 423
    Illegal PstI site found at 435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1766
    Illegal SapI site found at 683


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Categories
Parameters
None